Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 7pg1
RFLP LINKAGE MAPPING WITH cDNA CLONE MARKERS IN RICE.
Kimiko Yamamoto, Jianzhong Wu, Baltazar A. Antonio, Yoshiaki
Harushima, Yoshiaki Nagamura, Eiji Domon, Ayahiko Shomura,
Takamichi Toyama, Yasuaki Tamura, Takehiko Shimizu, Norio
Sue, Yoshiki Miyamoto, Zi Xuan Wang, Isamu Ohta, Maki Hori,
Eriko Matsui, Jianyu Song, Takuji Sasaki, Nori Kurata and
Yuzo Minobe, Rice Genome Research Program, National Institute
of Agrobiological Resources and Institute of the Society for
Techno-innovation of Agriculture, Forestry and Fisheries,
Kannondai, Tsukuba, lbaraki, 305 Japan
To study the structure of rice genome, a close RFLP
mapping is performed in F2 population derived from a cross
between Nipponbare, a japonica variety, and Kasalath, an indica
variety. As the probes for the mapping, we use cDNA clones from
mRNAs in callus of Nipponbare in addition to genomic clones. The
usage of cDNA clones for RFLP markers appears to be advantageous
in the following points: 1) the frequency of polymorphism
detected with cDNA probes is much higher than that with genomic
DNA probes (about 65% of the cases shows RFLP when chromosomal
DNA is digested with eight enzymes); 2) the sites of functional
genes can be mapped; 3) by using previously characterized cDNA
markers, RFLP map can be correlated with the conventional genetic
linkage map composed by phenotypic markers; and 4) since all
cDNA probes having been mapped are sequenced partially, the map
tagged with sequence information can be constructed. The results
we obtained show that the cDNA mapping is a powerful tool
analyzing genome structure and function.
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