Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 76pg1
RFLP MAP CONSTRUCTION IN SORGHUM AND COMPARATIVE MAPPING IN
MAIZE.
M. G. Pereira 1, M. Lee 1, P. Bramel-Cox 2, and W. L. Woodman 1.
l Department of Agronomy, Iowa State University, Ames, IA
50011,and 2 Department of Agronomy, Kansas State University,
Manhattan, KS 66502.
The first RFLP linkage map in Sorghum based on CDNA probes
has been developed. Probes were mapped in an F2 population
represented by 78 individuals of the cross, CK60 x P1229828.
Probes were derived from libraries of maize cDNAs (160) and
sorghum genomic PstI fragments (19), and collections of mapped
maize genomic clones (42) selected for their ability to produce
single-low copy, polymorphic hybridization patterns with sorghum.
The cDNA probes provided much stronger signal intensity and
detected higher frequencies of polymorphism with HindIII and
EcoRI than the maize genomic probes. The probes defined 203 loci
placed into 10 linkage groups, 1756 cM. Eighty percent of the
markers linked in maize were also linked in sorghum. Parents
used to create the mapping populations are extremely divergent
for vegetative and reproductive morphology, resistance to
greenbug (Schizapus graminum) and sorghum downy mildew
(Peronosclerospora sorghi), and maturity. Studies to locate
genes controlling these traits in sorghum, compare their map
positions in maize, and to asses conservation of gene order
between sorghum and maize have been initiated.
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