Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 6pg1
MOLECULAR TECHNIQUES AND STATISTICAL ANALYSES FOR TYPING AND
CHARACTERIZATION OF PLANT INTRASPECIFIC VARIATION.
Stephen Kresovich and Warren F. Lamboy, USDA-ARS,
Plant Genetic Resources Unit, Cornell, University,
Geneva, NY 14456-0462.
The goal of this research is to establish strategies and
techniques to detect and quantify intraspecific variation of
plants at the molecular level. Theories and techniques of
molecular and population genetics now may be readily integrated
for establishing measures of uniqueness and variation. We are
utilizing a battery of ploymerase chain reaction (PCR)-based
assays for both coding (AHAS and Cab multigene families) and
non-coding regions (simple, interspersed, moderately repetitive
DNA) of the genome to establish: (1) individual identification,
(2) measures of genetic variation, and (3) estimations of genetic
relatedness. Moreover, this information, also should advance our
understanding of useful genes and genotypes. We highlight
aspects of technique selection and optimization, cost
containment, data handling, and appropriate statistical analysis.
Linked with traditional approaches to measurement of
intraspecific variation, these newer methods will produce higher
resolution and more rapid characterization and evaluation of ex
situ and in situ plant populations. The resultant information
will greatly aid efforts in agricultural and conservation
sciences.
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