Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 56pg1
A GENETIC LINKAGE MAP OF Saccharum spontaneum 'SES208'
Salah M. Al-Janabi, Rhonda. J. Honeycutt, Michael McClelland, and
Bruno W.S. Sobral. California Institute of Biological Research
1099 N. Torry Pines Road, Suite 300, La Jolla, Ca, 92037
The Arbitrarily Primed Polymerase Chain Reaction (AP-PCR)
was used to construct a genetic linkage map of the genome of the
Saccharum spontaneum 'SES208'. The mapping population was
composed of 88 progeny from the ADP 85-0068 X SES 208 cross.
Nearly 300 primers (10-mers) were screened against the mapping
parents, SES208 and ADP068. Approximately 100 of the 300 primers
were selected for mapping based on number of polymorphism
detected that distinguished the mapping parents. Using these
primers we screened more than 1600 loci, of which 300 were
polymorphic. Over 230 of these polymorphic fragments segregated
1:1 (at 98% confidence level) in the progeny, and therefore could
be mapped using Map Maker. The three point analysis of the
mapping data using maximum theta of 0.25 and minimum LOD score of
3.0 showed that 20% of the markers are unlinked, while 80% of the
markers are in linkage groups comprised of 2 or more markers.
Linkages were also detected in the joint map that combines the
results of both AP-PCR and the RFLP polymorphisms detected in the
same population by a group at Cornell University.
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