Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 55pg1
RECONSTRUCTING HISTORIES OF MAIZE INBREDS USING MOLECULAR
CHARACTERS.
Rhonda Honeycutt 1, Stephen Smith 2, and Bruno Sobral 1, 1
California Institute of Biological Research, 11099 North Torrey
Pines Road, Ste. 300, La Jolla, CA 92037 2 Pioneer Hi-bred
International, Plant Breeding Division, 7250 NW 62nd Avenue,
Johnston, IA 50131-1004.
In crop species, phylogenetic relationships have been
assessed on the basis of morphological, cytological, isozyme,
flavonoid, or restriction fragment length polymorphism (RFLP)
characters. We are using the arbitrarily primed polymerase chain
reaction (AP-PCR) to generate molecular fingerprints of maize
inbred lines in order to investigate statistical methods that
most correctly reconstruct the known pedigree. In a blind study,
15 primers (10-mers) were used to generate over 100 AP-PCR
characters scored for each of 22 inbred lines. In addition, 456
characters were generated using a total of 99 mapped RFLP probes
that collectively provided a sampling along each chromosome arm
for the same group of inbred lines. Here we present the analyses
of these datasets independently and collectively using both
phenetic and cladistic methods.
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