PAG-I Plant Genome I Conference

Town & Country Conference Center, San Diego, CA, November, 1992.


PG-I: 48pg1

PARAMETERS AFFECTING THE POWER OF INTERVAL MAPPING

William D. Beavis, DMD, Pioneer Hi-Bred Int'l.


Considerable effort has been devoted to understanding the LOD threshold needed to avoid false positive identification of quantitative trait loci (QTL). Based on this threshold, experimental results from F2 derived populations have been nconsistent. Most comparisons of QTL have beenamong populations, thus inconsistent results may have genetic explanations. However, several researchers have studied F2 derived lines from B73xM017 and also have obtained inconsistent results. This prompted a simulation study of the parameters affecting the power of interval mapping to consistently detect real QTL. For a LOD threshold associated with a genome size of 1500 cM, and a genetic model of simple additive genetic effects, I studied the impact of sample size (number of F2 derived lines), number and magnitude of QTL effects, heritability, and density of co-dominant genetic markers on the power function. The results suggest that most experimental results to date have been based on inadequate sample sizes for all table traits with few segregating QTL. Also, these results provide an explanation for the inconsistent identification of QTL.


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