Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 46pg1
A PARTIAL GENETIC LINKAGE MAP OF SLASH PINE (PINUS ELLIOTTII
ENGELM. VAR. ELLIOTTII) BASED ON RANDOMLY AMPLIFIED POLYMORPHIC
DNAS.
C.D.Nelson, W.L. Nance, and R.L. Doudrick. U.S.D.A. Forest
Service, Southern Forest Experiment Station, Forestry Sciences
Laboratory, Gulfport, MS.
A set of 420 random, 10-base--oligonucleotide primers was
screened for repeatable, polymorphic (RAPD) bands within 8
megagametophytes from a single slash pine (Pinus elliottii
Engelm. var. elliottii) tree. The apparently repeatable and
polymorphic bands were further characterized within a larger
sample of 68 megagametophytes from the same tree. Bands
segregating in a 1:1, present-to-absent, ratio were classified as
heterozygous genetic markers and mapped using multi-point linkage
analysis. The analysis revealed 13 linkage groups of at least 3
loci, ranging in size of 28 to 68 cM, and 9 linked pairs of loci.
The 22 groups included 73 markers and covered approximately 782
cM of genetic mapping distance. Genome size estimates, based on
the linkage data, ranged from 28.8 to 33.6 M. When the 24
unlinked markers and the ends of the 13 linkage groups and 9
linked pairs were included, the set of RAPD markers covered
approximately 21.6 M or 64 to 75% of the genome. This extent of
genomic coverage should allow for the efficient mapping of genes
for reaction to the pathogenic fungus, Cronartium quercumm f. sp.
fusiforme.
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