Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 45pg1
GENETIC ANALYSIS OF A SINGLE UREDINIOSPORE DERIVED CULTURE OF
CRONARTIUM QUERCUUM F. SP. FUSIFORME, USING RAPD MARKERS.
R. L. Doudrick, C. D. Nelson, and W. L. Nance. U. S. D. A. Forest
Service, Southern Forest Experiment Station, Forestry Sciences
Laboratory, Gulfport, MS.
Random amplified polymorphic-DNA (RAPD) markers were
developed for Cronartium quercuum f. sp. fusiforme, the causal
agent of fusiforme rust disease on southern pines. Genetic
linkages were identified between 103 RAPD marker loci in a
segregating population of 48 gametothalli and drops of spermatia
produced from a single urediniospore derived culture. A
preliminary genetic linkage map with eight linkage groups and 12
linked pairs is presented. Based on the extent of linkage
detected among the loci identified, the size of the nuclear
genome of C.q. fusiforme is estimated to be approximately 5100
recombination units (centiMorgans, cM). The 103 loci
characterized provide approximately 40% coverage of the genome
at 25 cM separation between loci. The RAPD marker loci may be of
immediate utility in fingerprint identification of laboratory
cultures and field isolates for epidemiological studies. Even
this preliminary genetic linkage map has great potential for
aiding in studies on host:pathogen interactions in fusiform rust
disease and monitoring variation in pathogen populations.
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