Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: 39pg1
ANALYSIS AND MANIPULATION OF THE TOMATO GENOME USING A HIGH
DENSITY MOLECULAR LINKAGE MAP.
S.D. Tanksley, M.W. Bonierbale, P. Broun, M.W. Ganal, G.B.
Martin, A.H. Paterson, J.P. Prince, and M.C. de Vicente,
Department of Plant Breeding and Biometry, 252 Emerson Hall,
Cornell University, Ithaca, NY 14853.
More than 1000 markers (mainly RFLPS) have been mapped onto
the tomato molecular linkage map and these markers cover a total
of 1276 map units. Many of these markers have also been mapped
onto the potato and pepper linkage maps making it possible to
determine chromosomal homeologies with a fairly high degree of
precision. Several of the tomato telomeres have also been mapped
using a telomere-associated tandemly repeated DNA sequence
(TGRI). Thus far all of the telomeres map close to the most
distal RFLP markers on the linkage map indicating that telomeres
do not experience excessive rates of recombination. In contrast,
areas around centromeres experience a 10 to 100-fold reduction in
recombination. The tomato molecular linkage map is being used in
a number of research areas including: 1) population/evolutionary
studies 2) gene tagging/identification 3) map-based cloning.
Highlights of results from the first two areas will be covered in
this presentation.
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