PAG-I Plant Genome I Conference

Town & Country Conference Center, San Diego, CA, November, 1992.


PG-I: 37pg1

LINKAGE GROUP COMPARISON FOR THE A, B, AND C GENOMES OF THE GENUS BRASSICA

M.J. Truco and C.F. Quiros. Department of Vegetable Crops. University of California. Davis, CA 95616.


In order to study the evolution of Brassica genomes, we compared linkage maps of the three diploid species in this genus: B. rapa (A genome), B. nigra (B genome) and B. oleracea (C genome). The linkage maps were generated from F2 segregating populations using the same set of probes (random and known sequences from a cDNA library of B. napus, SI gene from B. oleracea and seed storage protein genes). Some isozymes were also included. Only 40% of the probes were segregating in all three populations, the rest were polymorphic only in two of the populations simultaneously. It was possible to find conserved regions between each pair of linkage maps, but in general there was a lot of reordering among the genomes. There were more conserved regions between B. rapa and B. oleracea linkage maps than between any of these two species and B. nigra linkage map. This finding agrees with other reports suggesting a closer phylogenetic relationship between B. rapa and B. oleracea than between these species and B. nigra.


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