Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: CENTROMERE TO RFLP MARKER DISTANCES USING A DITELOSOMIC LINE OF
SUN II OAT
CENTROMERE TO RFLP MARKER DISTANCES USING A DITELOSOMIC LINE OF
SUN II OAT.
Bai-chai Wu, Shahryar F. Kianian, Ronald L. Phillips and Howard
W. Rines, Department of Agronomy and Plant Genetics, University
of Minnesota, St. Paul, NW 55108.
Ditelosomic line DIX of 'SUN II' oat (Avena sativa L.) was
crossed with euploid 'Kanota' to derive populations for mapping
RFLP markers in relation to the centromere. Chromosomal
constitutions of F2 plants and plants from reciprocal crosses
between chromosomally heteromorphic F1s and the Kanota parent
were determined by cytological observation and confirmed by
analysis of a rDNA RFLP sequence that maps to the short arm of
chromosome IX. RFLP markers placed on the respective chromosome
arms by nullisomic and ditelosomic analysis were used for
mapping. Recombination values in the long arm of chromosome IX
were obtained by analyzing the chromosome constitutions and RFLP
patterns of the progeny populations. Transmission frequencies of
telosomic male and female gametes were 0.21 and 0.27,
respectively. Six RFLP markers, UMN 30, UMN 149, UMN 214, UMN
283, UMN 753 and CDO 309, were mapped to the long arm of 'Sun II'
DIX. UMN 30 and UMN 149 showed similar bias in segregation ratio
as the telosome transmission frequency indicating that they were
linked to the centromere. UMN 214, UMN 283, UMN 753 and CDO 309
were linked to each other but independent of the centromere. The
combined map distance of the long arm of chromosome IX is at
least 100 map units.
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