Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: SYNTHETIC RFLP MARKERS USED TO ESTIMATE GENETIC RECOMBINATION
IN INTRA- AND INTERSPECIFIC CROSSES OF HEXAPLOID OAT (Avena
species, 2n=6x=42)
SYNTHETIC RFLP MARKERS USED TO ESTIMATE GENETIC RECOMBINATION
IN INTRA- AND INTERSPECIFIC CROSSES OF HEXAPLOID OAT (Avena
species, 2n=6x=42).
Shahryar F. Kianian, Ronald L. Phillips, and Howard W. Rines,
Department of Agronomy and Plant Genetics, Univ. of Minnesota
and USDA-ARS., St.Paul, MN 55108.
Avena sterilis has been used extensively to improve
cultivated oat. Recombination in interspecific crosses with A.
sativa may be reduced, based on cytological and breeding
observations. In this study we are investigating possible
correlations between meiotic irregularities in F, hybrids of oat
and distortions in segregation ratios and linkage alterations in
F2 progenies. Meiotic analysis of F, intra- and interspecific
hybrids of A. sativa and A. sterilis revealed a frequent
occurrence of reciprocal translocation(s) between various
accessions. The number of precocious chromosomes in metaphase I
of meiosis in these hybrids was highly correlated with that in
metaphase IL and the number of lagging chromosomes in anaphase I
and anaphase R was highly correlated with micronuclei frequency
in tetrads. RFLP analysis of DNA from nullisomic and ditelosomic
lines was used to assign 56 markers to nine syntenic groups
(sequences mapping to the same physical chromosome). Potentially
linked markers identified by this method are being used to
measure recombination in F2 intra- and interspecific progenies.
A translocation involving two chromosomes has been identified in
the interspecific populations with these markers.
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