Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: RFLP AND RAPD MAPPING OF Eucalyptus globulus
RFLP AND RAPD MAPPING OF Eucalyptus globulus
Yonggen Song and Christopher A. Cullis; Department of Biology,
Case Western Reserve University, Cleveland, OH 44106
A successful high resolution-mapping effort will provide a
major input toward the understanding of genetics and breeding of
Eucalyptus. In the present study, we used both RFLP and RAPD
markers to construct the linkage map of E.globulus. Thirty seven
F1 individuals derived from an intraspecific cross of individual
trees designated C33 x MulO were employed for the purpose. In an
outbreeding species such as Eucalyptus, both parents should be
heterozygous at many loci, therefore, the segregation of
polymorphic fragments could be determined. The RFLP markers were
obtained from size-selected Pst I clones; whereas, the RAPD
markers were produced by using PCR, surveyed with one hundred
10-mer single arbitrary primers, as described by Williams et al.
(1990)[1]. The data were analyzed using Linkage-l program
(Suiter et al. 1987)[2]. The distribution of all loci obtained
over the Eucalyptus chromosomes reveal a maximum number for
chromosome designated 3E and a minimum for chromosome designated
8E. In addition, the study was extended to investigate variation
among five species of Eucalyptus using the Pst I clones and
several heterologous probes. The amount of polymorphism among
the interspecific progeny exceeded that of the intraspecific
progeny. We suggest that the markers found in the study may be
useful in accelerated breeding program for economically important
characters such as wood quality and cold tolerance.
1. Williams, J. G. K., Kubelik, A. E., Levak, K. J., Rafalski, J.
A. and Tingey, S.C. 1990. Nucleic Acids Res 18: 6531-6535. 2.
Suiter, K. A., Wendel, J. F. and Case, J. S. 1987. Linkage-]
(version 3.50) User's Manual. North Carolina State University,
Raleigh, N.C.
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