Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: IDENTIFICATION OF RAPD POLYMORPHISMS AMONG INTROGRESSED FLAX
LINES CONTAINING THE M RUST RESISTANCE GENES, AND THE CLONING OF
THE PCR PRODUCTS
IDENTIFICATION OF RAPD POLYMORPHISMS AMONG INTROGRESSED FLAX
LINES CONTAINING THE M RUST RESISTANCE GENES, AND THE CLONING OF
THE PCR PRODUCTS.
Chris Cullis and Didahally Govindaraju, Biology Department, Case
Western Reserve University, Cleveland, OH 44106.
Most of the flax rust resistance genes have been
incorporated into a Bison background by Flor. A series of 7
lines, each containing a single M gene, was developed by
backcrossing to Bison 8 to 14 times. These lines and the source
parents along with Bison were used in a comparison to identify
RAPD polymorphisms based on screening with 100 random 10-mers as
primers. A number of reproducible polymorphisms were identified
between the introgressed lines and Bison. The polymorphic
pattern was checked against the relevant source parent to ensure
that the difference was consistent with it being derived from the
source parent. The most polymorphisms were observed with the
line which had only been backcrossed to Bison 8 times compared to
those backcrossed 12 or 14 times. The polymorphic bands from
Bison and/or the differential were excised and cloned using the
TA cloning system from Invitrogen. The cloned amplified
fragments will be used to test whether or not they highlight
useful RFLPS. In addition, random PCR amplification products
have been cloned to determine the relative frequency of
repetitive and low copy sequences being amplified in flax using
random 10-mers as primers.
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