Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: AUTOMATED NUCLEIC ACID PURIFICATION OF PLANT TISSUE
AUTOMATED NUCLEIC ACID PURIFICATION OF PLANT TISSUE
Sandy M. Koepf, Takashi Fukushima and Criss Lamb, Applied
Biosystems Inc., 850 Lincoln Centre Dr., Foster City, CA 94404.
Manual nucleic acid extraction is a time consuming
procedure. There can be variability in the yield and quality of
the nucleic acid recovered depending on the procedure and type of
starting material. When the starting material is plant tissue,
variability is even higher due to secondary metabolites which
maybe present. Some secondary metabolites can co-purify with the
nucleic acid. We have observed that high levels of secondary
metabolites are associated with inhibition of restriction
digestion of the DNA or the degradation of the nucleic acid. We
have focused on ways to make nucleic acid purification of plant
tissue more robust. One way was to automate the purification
steps l by using the 341 GENEPURE (TM) Nucleic Acid Purification
System by Applied Biosystems. This automated method has
eliminated the run to run variability. To minimize the effects
of secondary metabolites, we have investigated different
chemistries on the 341.
Return to Previous Page or Intl-PAG Homepage