Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: GENETIC MAPPING OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)
MARKERS IN CITRUS
GENETIC MAPPING OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)
MARKERS IN CITRUS.
Qinyin Cai, Charles Guy, and Gloria A. Moore, Departments of
Environmental and Horticultural Sciences, Institute of Food and
Agricultural Sciences, University of Florida, Gainesville, FL
32611.
Genetic mapping with RAPD markers has been initiated in
Citrus. Reproducible polymorphism of amplified DNA fragments has
been revealed with approximately half of the 60 - random primers
(obtained from Operon Technologies Inc., Alameda CA) thus far
tested. Apparent segregating loci revealed with these primers
are being analyzed for reproducibility, inheritance, and linkage
using 66 BC, progeny from an intergeneric cross of Citrus grandis
(L.) Osb. x [Citrus grandis (L.) Osb. x Poncirus trifoliata (L.)
Raf.]. This progeny population has previously been used to map
57 isozyme and RFLP markers [Durham et al., Theor. Appl. Genet.,
84, 39(1992)]. A current map will be presented. We hope to use
a highly populated linkage map to map quantitative trait loci for
cold tolerance in Citrus. To this end, each BC, progeny plant
from the cross between very cold-sensitive C. grandis and
extremely cold-hardy P. trifoliata is being propagated and will
ultimately be tested for response to cold acclimation. As a part
of this project, we have thus far isolated five unique
cold-acclimation responsive cDNA clones through differential
screening from a library constructed from cold acclimated tissue
of P. trifoliata. Work is in progress to determine whether
these clones can be placed on the linkage map.
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