Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: A COMPARISON OF MALE AND FEMALE RECOMBINATION RATES IN WHEAT
USING RFLP MAPS OF HOMOEOLOGOUS GROUP 6 AND 7 CHROMOSOMES
A COMPARISON OF MALE AND FEMALE RECOMBINATION RATES IN WHEAT
USING RFLP MAPS OF HOMOEOLOGOUS GROUP 6 AND 7 CHROMOSOMES.
Wang Gang, Gina Hyne, Shiaoman Chao, Mike Gale and John Snape,
Cambridge Laboratory, JI Centre for Plant Science Research,
Colney Lane, Norwich, UK.
Separate populations of doubled haploid lines were developed
from male and female gametes of a cross between two single
chromosome substitution lines of bread wheat, Highbury (Chinese
Spring 5B) and Sicco (Chinese Spring 5B), where the 'male'
population was derived using anther culture and the 'female'
population using the Hordeum bulbosum system. RFLP and isozyme
markers polymorphic in this cross for chromosomes of homoeologous
groups 6 and 7 were then used to develop separate 'male' and
'female' genetic maps. Maps were compared and revealed that gene
order was the same on both maps and that pairwise recombination
frequencies were not significantly different for any chromosome
or linkage group. These first results suggest that there are no
significant differences in male and female recombination
frequencies in bread wheat, and apparently little environmental
influence, since the populations were developed under different
environmental conditions.
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