Plant Genome I Conference
Town & Country Conference Center, San Diego, CA, November, 1992.
PG-I: RFLP ANALYSIS OF A SOYBEAN BREEDING POPULATION: GENETIC
STRUCTURE AND TRAIT VARIATION
RFLP ANALYSIS OF A SOYBEAN BREEDING POPULATION: GENETIC
STRUCTURE AND TRAIT VARIATION.
P. Keim, W. Beavis, J. Schupp, R. Freestone, M.
Vahedian, D.M. Webb and T. Gocken. Northern Arizona
Univ., Flagstaff AZ 86011-5640 and Pioneer Hi-Bred Int.,
Johnston IA 50131
Molecular marker analysis has been difficult to use in
adapted soybean germplasm because of relatively low levels of
diversity. We have exhaustively surveyed 16 cultivars to
identify ca. 700 informative RFLP markers. Two of these
cultivars had been used to construct a segregating population
consisting of 261 F5-derived lines. One-hundred-fifty-five RFLP
markers were scored in this population and used for estimating
genetic distances (GDR) among the segregating lines. While the
genetic composition of individual lines met theoretical
expectations, the frequency distribution of GDR estimates was
significantly skewed towards zero. This is consistent with
repetitive sampling of individual genotypes during inbreeding.
Redundant genotypes could then be eliminated from subsequent
genetic analyses. Genetic linkage groups among marker loci was
determined using MAPMAKER. Trait variation in this population
was quantified by replicated field trial at four locations in two
years. Marker loci were used to identify regions of the genome
that contain QTLs affecting agronomic traits.
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